HPLC Column, RP C4 Butyl, Wide Pore, 3um, 2.1mm ID x 100mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4 Butyl™ Wide Pore is a reversed-phase HPLC stationary phase specifically designed for the separation of proteins, peptides, enzymes, and other large biomolecules. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, this 300 Å wide pore chemistry provides enhanced accessibility for larger molecules compared to standard 100 Å pore columns. The wide pore structure improves mass transfer and interaction with the stationary phase, making it an excellent choice for bioanalytical, biopharmaceutical, and protein characterization applications.
68304-10P-2-3M is an HPLC column packed with 3 µm RP C4 Butyl™ Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 100 mm format. This narrow-bore configuration combines increased resolution with reduced solvent consumption and excellent compatibility with LC-MS instrumentation. The 100 mm length provides greater peak capacity and resolving power than shorter 50 mm columns, while the 3 µm particles deliver higher efficiency and sharper peaks than comparable 5 µm packings. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, any HPLC system used with this column must be capable of precise and reproducible low-flow operation.
This column is particularly well suited for proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules analyzed by reversed-phase HPLC or LC-MS. The C4 chemistry provides lower hydrophobic retention than C8 phases, which is often advantageous for biomolecules that may be excessively retained on more hydrophobic stationary phases.
Key Benefits
RP C4 Butyl™ Wide Pore stationary phase
Fully double end capped
Traditional butyl (C4) bonded phase
Ultra-high purity silica support
300 Å wide pore design
Optimized for proteins and peptides
3 µm particle technology
Greater efficiency than 5 µm columns
Ideal for LC-MS methods
Reduced solvent consumption
Typical Applications
Protein analysis
Peptide analysis
Enzyme characterization
Bioanalytical methods
LC-MS methods
Biopharmaceutical analysis
Protein purity testing
Peptide mapping
Biomolecule separations
Method development
Specifications
Product Type: HPLC Column
Stationary Phase: RP C4 Butyl™ Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 2.1 mm ID x 100 mm
Length: 100 mm
Typical Flow Rate: 0.2–0.3 mL/min
Brand: Cogent
Important Notes
Designed for proteins, peptides, enzymes, and other large biomolecules
300 Å wide pores improve access to the bonded phase for larger molecules
Suitable for conventional HPLC and LC-MS systems
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Generates higher backpressure than larger particle columns
The 2.1 mm ID format is commonly used for LC-MS applications
Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min
Detector flow cells should be optimized for low-volume, narrow-bore chromatography
The 100 mm length provides greater resolution and peak capacity than 50 mm columns
Lower retention than comparable C8 stationary phases